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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
28/03/2011 |
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Data da última atualização: |
29/04/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
OLIVEIRA, T. M.; PIROVANI, C. P.; COSTA, M. G. C.; SOARES FILHO, W. dos S.; GESTEIRA, A. S. |
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Afiliação: |
T . M. Oliveira, UESC; Carlos Priminho Pirovani, UESC; Marcio Gilberto Cardoso Costa, UESC; WALTER DOS SANTOS SOARES FILHO, CNPMF; ABELMON DA SILVA GESTEIRA, CNPMF. |
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Título: |
Identification of proteins involved in response mechanisms to water stress in citrus. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
pdf 34710 |
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Conteúdo: |
The citrus industry is an important economic sector worldwide, with Brazil being the world?s largest producer and exporter of orange juice frozen concentrated orange. In Brazil, among the abiotic stresses, drought is one that most often negative impact on productivity of citrus. Different families proteins are known to be associated with plant responses to stresses abiotic, again being synthesized, as well as having their concentrations accumulated or diminished. This proposal aims to identify key proteins that are related to responses to drought stress in tolerant genotypes of citrus and likely forced into this position. Therefore, two contrasting varieties of citrus, Rangpur lime and tangerine Sunki were subjected to two water regimes: (i) Control: plants grown in field capacity and (ii) water stress, plants grown without irrigation. The changes in protein dynamics in the leaves and roots were analyzed at three different times during water stress. Proteins were extracted using the method of ADP (Acetone Power Dry), followed by SDS-dense phenol. The quantification was done using the 2-D Quantification kit. Protein both treatments were extracted in triplicate and separated using SDS-PAGE bi-dimensional 12%. The analysis of 2D maps showed about 300 spots per gel, showing the differentially expressed proteins. The proteins were identified in the MS Q-TOF and were classified into several categories, including protein related to photosynthesis, proteins involved in metabolism and production of energy, proteins related to metabolism of carbohydrates and proteins unknown biological function. Identifying these proteins will help in understanding of the biochemical processes involved in the mechanisms of response to drought and provide input to the development of rootstocks drought tolerant, so use this information to improve this important group of plants through genetic manipulation. MenosThe citrus industry is an important economic sector worldwide, with Brazil being the world?s largest producer and exporter of orange juice frozen concentrated orange. In Brazil, among the abiotic stresses, drought is one that most often negative impact on productivity of citrus. Different families proteins are known to be associated with plant responses to stresses abiotic, again being synthesized, as well as having their concentrations accumulated or diminished. This proposal aims to identify key proteins that are related to responses to drought stress in tolerant genotypes of citrus and likely forced into this position. Therefore, two contrasting varieties of citrus, Rangpur lime and tangerine Sunki were subjected to two water regimes: (i) Control: plants grown in field capacity and (ii) water stress, plants grown without irrigation. The changes in protein dynamics in the leaves and roots were analyzed at three different times during water stress. Proteins were extracted using the method of ADP (Acetone Power Dry), followed by SDS-dense phenol. The quantification was done using the 2-D Quantification kit. Protein both treatments were extracted in triplicate and separated using SDS-PAGE bi-dimensional 12%. The analysis of 2D maps showed about 300 spots per gel, showing the differentially expressed proteins. The proteins were identified in the MS Q-TOF and were classified into several categories, including protein related to photosynthesis, proteins involved in metabolism an... Mostrar Tudo |
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Palavras-Chave: |
Differential expression; Drought stress; Proteomic; Rootstock. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02637nam a2200217 a 4500
001 1883133
005 2011-04-29
008 2011 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, T. M.
245 $aIdentification of proteins involved in response mechanisms to water stress in citrus.
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.$c2011
500 $apdf 34710
520 $aThe citrus industry is an important economic sector worldwide, with Brazil being the world?s largest producer and exporter of orange juice frozen concentrated orange. In Brazil, among the abiotic stresses, drought is one that most often negative impact on productivity of citrus. Different families proteins are known to be associated with plant responses to stresses abiotic, again being synthesized, as well as having their concentrations accumulated or diminished. This proposal aims to identify key proteins that are related to responses to drought stress in tolerant genotypes of citrus and likely forced into this position. Therefore, two contrasting varieties of citrus, Rangpur lime and tangerine Sunki were subjected to two water regimes: (i) Control: plants grown in field capacity and (ii) water stress, plants grown without irrigation. The changes in protein dynamics in the leaves and roots were analyzed at three different times during water stress. Proteins were extracted using the method of ADP (Acetone Power Dry), followed by SDS-dense phenol. The quantification was done using the 2-D Quantification kit. Protein both treatments were extracted in triplicate and separated using SDS-PAGE bi-dimensional 12%. The analysis of 2D maps showed about 300 spots per gel, showing the differentially expressed proteins. The proteins were identified in the MS Q-TOF and were classified into several categories, including protein related to photosynthesis, proteins involved in metabolism and production of energy, proteins related to metabolism of carbohydrates and proteins unknown biological function. Identifying these proteins will help in understanding of the biochemical processes involved in the mechanisms of response to drought and provide input to the development of rootstocks drought tolerant, so use this information to improve this important group of plants through genetic manipulation.
653 $aDifferential expression
653 $aDrought stress
653 $aProteomic
653 $aRootstock
700 1 $aPIROVANI, C. P.
700 1 $aCOSTA, M. G. C.
700 1 $aSOARES FILHO, W. dos S.
700 1 $aGESTEIRA, A. S.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas; Embrapa Unidades Centrais. |
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Data corrente: |
19/09/1997 |
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Data da última atualização: |
27/06/2011 |
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Autoria: |
RESENDE, M. D. V. de; SOUZA JÚNIOR, C. L. de. |
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Afiliação: |
MARCOS DEON VILELA DE RESENDE, CNPF; CLAUDIO LOPES DE SOUZA JÚNIOR. |
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Título: |
Número de repetições e tamanho da parcela para seleção de progênies de milho em solos sob cerrado e fértil. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Pesquisa Agropecuaria Brasileira, Brasilia, v. 32, n. 8, p. 781-788, ago. 1997. |
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Idioma: |
Português |
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Conteúdo: |
O objetivo neste trabalho foi obter informacoes relativas a tamanho de parcela e numero de repeticoes para avaliacao de progenies de milho (Zea mays L.) em solo sob cerrado e em solo fertil. Utilizaram-se, como material experimental, 200 progenies de meios-irmaos da variedade BR 108. Aplicou-se um metodo que tem por base a diminuicao da variancia fenotipica entre medias de progenies e consequente aumento no ganho genetico. O aumento do numero de repeticoes mostrou-se mais eficiente que o aumento do tamanho da parcela para elevar o ganho. O aumento do numero de repeticoes conduziu a acrescimos similares no ganho genetico no cerrado e no solo fertil, e o aumento do numero de plantas por parcela conduziu a um maior acrescimo no ganho genetico no cerrado que no fertil, quanto ao caracter peso de espigas. Quanto ao caracter peso de espigas, indica-se a utilizacao de tres repeticoes com 20 plantas por parcela no cerrado, e tres repeticoes com 15 plantas por parcela no solo fertil. No tocante a altura da planta e da espiga, indica-se a utilizacao de tres repeticoes com tres plantas por parcela no cerrado, e tres repeticoes e cinco plantas por parcela no solo fertil. |
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Palavras-Chave: |
Acid soil; Experimental precision; Experimental technique; Genetic process; Maize; Precisao experimental; Progresso genetico; Tecnica experimental. |
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Thesagro: |
Milho; Solo Ácido; Zea Mays. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/36801/1/Pab1481.doc
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/44975/1/NUMERO-DE-REPETICOES-E-TAMANHO-DA-PARCELA.pdf
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Marc: |
LEADER 01984naa a2200265 a 4500
001 1309659
005 2011-06-27
008 1997 bl uuuu u00u1 u #d
100 1 $aRESENDE, M. D. V. de
245 $aNúmero de repetições e tamanho da parcela para seleção de progênies de milho em solos sob cerrado e fértil.
260 $c1997
520 $aO objetivo neste trabalho foi obter informacoes relativas a tamanho de parcela e numero de repeticoes para avaliacao de progenies de milho (Zea mays L.) em solo sob cerrado e em solo fertil. Utilizaram-se, como material experimental, 200 progenies de meios-irmaos da variedade BR 108. Aplicou-se um metodo que tem por base a diminuicao da variancia fenotipica entre medias de progenies e consequente aumento no ganho genetico. O aumento do numero de repeticoes mostrou-se mais eficiente que o aumento do tamanho da parcela para elevar o ganho. O aumento do numero de repeticoes conduziu a acrescimos similares no ganho genetico no cerrado e no solo fertil, e o aumento do numero de plantas por parcela conduziu a um maior acrescimo no ganho genetico no cerrado que no fertil, quanto ao caracter peso de espigas. Quanto ao caracter peso de espigas, indica-se a utilizacao de tres repeticoes com 20 plantas por parcela no cerrado, e tres repeticoes com 15 plantas por parcela no solo fertil. No tocante a altura da planta e da espiga, indica-se a utilizacao de tres repeticoes com tres plantas por parcela no cerrado, e tres repeticoes e cinco plantas por parcela no solo fertil.
650 $aMilho
650 $aSolo Ácido
650 $aZea Mays
653 $aAcid soil
653 $aExperimental precision
653 $aExperimental technique
653 $aGenetic process
653 $aMaize
653 $aPrecisao experimental
653 $aProgresso genetico
653 $aTecnica experimental
700 1 $aSOUZA JÚNIOR, C. L. de
773 $tPesquisa Agropecuaria Brasileira, Brasilia$gv. 32, n. 8, p. 781-788, ago. 1997.
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Registro original: |
Embrapa Florestas (CNPF) |
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